Difference between revisions of "Part:BBa K1773019"

Latest revision as of 14:09, 11 September 2015

Cas3 gene with pLux/cI right promoter and strong RBS

Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. We wanted to have different expression strengths of Cas3, so we cloned it also to a Medium RBS (BBa_K1773020), and a weak RBS (BBa_K1773021)

In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2722
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1443
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2406
Illegal AgeI site found at 2779
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1773019 short</partinfo>
-Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.
+Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. We wanted to have different expression strengths of Cas3, so we cloned it also to a Medium RBS (BBa_K1773020), and a weak RBS (BBa_K1773021)
+In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.
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