Difference between revisions of "Part:BBa K1638033"

Revision as of 10:48, 11 September 2015

T25 domain of cyaA with cAMP-induced RFP generator

The T18 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG). When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the conjugated proteins associates, T25 and T18 associates too. This leads to the catalysation of the conversion of ATP to cAMP. The rise in cyclic AMP can trigger the expression of genes by using a cAMP-induced promotor. For this construct a reporter system based on RFP controlled by the promoter PcstA is used. The presence of red-fluorescent cells can be used to verify protein-protein interactions.

See BBa_K1638032 for the T18 domain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1851
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 712
Illegal AgeI site found at 824
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1638033 short</partinfo>
-coming
+The T18 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG). When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the conjugated proteins associates, T25 and T18 associates too. This leads to the catalysation of the conversion of ATP to cAMP.
+The rise in cyclic AMP can trigger the expression of genes by using a cAMP-induced promotor. For this construct a reporter system based on RFP controlled by the promoter PcstA is used. The presence of red-fluorescent cells can be used to verify protein-protein interactions.
+See <partinfo>BBa_K1638032</partinfo> for the T18 domain.
 <!-- Add more about the biology of this part here