Difference between revisions of "Part:BBa K1604000"

Revision as of 16:07, 10 September 2015

Strong RBS + Proteorhodopsin

Proteorhodopsin (PR) is a light-powered proton pump. It creates a proton motive force across the membrane that can subsequently power cellular processes, such as ATP synthesis.

Usage and Biology

We improved part BBa_K773002 of Caltech 2012 by adding a strong RBS. We placed BBa_K1604000 under an inducible promoter and we fully characterized proteorhodopsin.
To see the characterization results check the part BBa_K1604010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 529
Illegal XhoI site found at 102
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 291
Illegal AgeI site found at 666
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1604000 short</partinfo>
-AAA
+Proteorhodopsin (PR) is a light-powered proton pump. It creates a proton motive force across the membrane that can subsequently power cellular processes, such as ATP synthesis.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+We improved part <html><a href="https://parts.igem.org/Part:BBa_K773002">BBa_K773002</a></html> of Caltech 2012 by adding a strong RBS.
+We placed BBa_K1604000 under an inducible promoter and we fully characterized proteorhodopsin. <br>
+To see the characterization results check the part <html><a href="https://parts.igem.org/Part:BBa_K1604010">BBa_K1604010</a></html>.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1604000 SequenceAndFeatures</partinfo>