Difference between revisions of "Part:BBa K1686046"
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===Usage and Biology===
 
===Usage and Biology===
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Curdlan is a bacterial polysaccharide that has been of significant recent interest due to its interesting and valuable properties and its bioactivity.
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In fact crdS codes Curdlan Synthase.
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The crdS product (73 kDa) deduced from the DNA sequence (1,965 bp) is curdlan synthase. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout–Cin disposition
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The molecular genetics of curdlan production have been investigated in Agrobacterium sp. ATCC31749. Studies on transposon-insertion mutants with altered capacity to stain with aniline blue when grown on indicator agar identified four genes (crdA, crdS, crdC, crdR) that are essential for curdlan production
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The crdS gene of Agrobacterium sp. strain ATCC31749 encodes the curdlan synthase (CrdS) protein

Revision as of 15:41, 10 September 2015

Curdlan synthase gene with codon optimisation for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal AgeI site found at 1192
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Curdlan is a bacterial polysaccharide that has been of significant recent interest due to its interesting and valuable properties and its bioactivity.

In fact crdS codes Curdlan Synthase.

The crdS product (73 kDa) deduced from the DNA sequence (1,965 bp) is curdlan synthase. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout–Cin disposition

The molecular genetics of curdlan production have been investigated in Agrobacterium sp. ATCC31749. Studies on transposon-insertion mutants with altered capacity to stain with aniline blue when grown on indicator agar identified four genes (crdA, crdS, crdC, crdR) that are essential for curdlan production


The crdS gene of Agrobacterium sp. strain ATCC31749 encodes the curdlan synthase (CrdS) protein