Difference between revisions of "Part:BBa K1773003:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1773003 short</partinfo>
 
<partinfo>BBa_K1773003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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no
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Gene mutagenesis was performed using Invitrogen GeneArt® Site-Directed Mutagenesis PLUS System kit.
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These mutagenic primers were used:
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EcoRI mutagenesis: <br />
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F: cgttttcaatggcagaatccggcatttgatttggca<br />
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R: tgccaaatcaaatgccggattctgccattgaaaacg<br />
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XbaI Mutagenesis:<br />
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F: cgatcatcattattcttctctggatgctgatgttaatttggg<br />
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R: cccaaattaacatcagcatccagagaagaataatgatgatcg<br />
  
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PstI Mutagenesis:<br />
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F: gaagactgaactgcctgcggttaaacaacataaac<br />
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Re: gtttatgttgtttaaccgcaggcagttcagtcttc<br />
  
  
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===Source===
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Primers for adding Biobrick prefix and suffix:
  
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A.actinomycetemcomitans D7-S
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Fw: ttaaggaattcgcggccgcttctagatgaatatcttacttgttagccaatgccaaaaaaa
  
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===References===
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Rev: cggctgcagcggccgctactagtagagatcttcctgccaaaaacccagcgttt

Latest revision as of 15:11, 10 September 2015

I-F type CRISPR-Cas Cas3 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2623
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2307
    Illegal AgeI site found at 2680
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene mutagenesis was performed using Invitrogen GeneArt® Site-Directed Mutagenesis PLUS System kit.

These mutagenic primers were used:

EcoRI mutagenesis:
F: cgttttcaatggcagaatccggcatttgatttggca
R: tgccaaatcaaatgccggattctgccattgaaaacg

XbaI Mutagenesis:
F: cgatcatcattattcttctctggatgctgatgttaatttggg
R: cccaaattaacatcagcatccagagaagaataatgatgatcg

PstI Mutagenesis:
F: gaagactgaactgcctgcggttaaacaacataaac
Re: gtttatgttgtttaaccgcaggcagttcagtcttc


Primers for adding Biobrick prefix and suffix:

Fw: ttaaggaattcgcggccgcttctagatgaatatcttacttgttagccaatgccaaaaaaa

Rev: cggctgcagcggccgctactagtagagatcttcctgccaaaaacccagcgttt