Difference between revisions of "Part:BBa K1604020:Design"

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===Source===
 
===Source===
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Arabinose inducible araC-pBAD promoter come from E. coli genome.
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Arabinose inducible araC-pBAD promoter come from <i>E. coli</i> genome.
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Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome.
+
beta&; carotene gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome
  
 
===References===
 
===References===

Revision as of 14:57, 10 September 2015

araC-pBAD + beta-carotene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2721
    Illegal NgoMIV site found at 2851
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Standard assembly between BBA_K731201 and a PCR amplification of BBa_K274200. BBa_K274200 was extracted by the 2012 distribution kit in pSB1A2 and it was amplified by PCR to restore a missing SpEI site.


Source

Arabinose inducible araC-pBAD promoter come from E. coli genome. beta&; carotene gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome

References