Difference between revisions of "Part:BBa K1602005"

 
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        <h1>Xylose to xylitol converting operon</h1>
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        <b>Xylose</b> is a monosaccharide belonging to the aldopentose family. Through reduction it can be converted to xylitol. The reaction takes place in the cytosol of the host and recent studies show, that the formation of xylitol in E.coli seems possible as well.
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        To enable the reduction in <i>E.coli</i> it is mandatory to establish an operon containing the coding gene <i>GRE3</i> for a aldose reductase. The gene is taken from <i>Saccharomyces cerevisiae</i>. The aldose reductase converts xylose to xylitol in dependence of NADPH.
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        <!-- BILD XYLAN ZU XYLOSE
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        <img style="width: 900px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="pictures/chem_way.jpg">
  
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            <b>Figure 1</b> Reaction scheme of the xylan to xylose converting operon. Xylan is the only substrate needed for the reaction. Xylan is metabolized to xylose in 1 step.
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      ===<h2>Usage</h2>===
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            This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>) in front of it, under control of a T7 Promoter (<a href="/Part:BBa_I719005">BBa_I719005</a>).
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                        <li class="block-10vi">aldo reductase - GRE3                     <a href="/Part:BBa_K1602004">(BBa_K1602004)</a></li>
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                        <img style="width: 400px; height: 120px;" alt="" src="pictures/gene_operon.png" align="center">
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                    <b>Figure 2</b> Genetic map of the xylan to xylose converting operon with T7 promoter. This brick directs the flux towards and finally                            enables <i>E.Coli</i> BL21 cells to convert xylan to xylose in presence of the inductor IPTG.
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===<h2>Sequence and Features</h2>===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1602005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1602005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K1602005 parameters</partinfo>
 
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Revision as of 13:03, 10 September 2015

Xylose to xylitol converting operon

Xylose is a monosaccharide belonging to the aldopentose family. Through reduction it can be converted to xylitol. The reaction takes place in the cytosol of the host and recent studies show, that the formation of xylitol in E.coli seems possible as well.
To enable the reduction in E.coli it is mandatory to establish an operon containing the coding gene GRE3 for a aldose reductase. The gene is taken from Saccharomyces cerevisiae. The aldose reductase converts xylose to xylitol in dependence of NADPH.

Figure 1 Reaction scheme of the xylan to xylose converting operon. Xylan is the only substrate needed for the reaction. Xylan is metabolized to xylose in 1 step.



===

Usage

===

This part is a composite of one coding gene and a strong RBS (BBa_B0034) in front of it, under control of a T7 Promoter (BBa_I719005).


Figure 2 Genetic map of the xylan to xylose converting operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to convert xylan to xylose in presence of the inductor IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]