Difference between revisions of "Part:BBa K1618023"

Revision as of 14:36, 9 September 2015

GlgB-Rv3030 with IPTG-inducible promoter

Rv3030 is a gene which encodes for a putative acyltransferase which will hopefully acylate glycogen chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector. The bacteria therefore shows chloramphenicol resistance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 283
Illegal BamHI site found at 1491
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 917

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 __NOTOC__
 <partinfo>BBa_K1618023 short</partinfo>
-Rv3030 is a gene which codes for a putative acyltransferase which acylates glycogen/starch chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and reattach the cut glycogen strand and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector.
+Rv3030 is a gene which encodes for a putative acyltransferase which will hopefully acylate glycogen chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector. The bacteria therefore shows chloramphenicol resistance.
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