Difference between revisions of "Part:BBa K1773003:Design"
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<partinfo>BBa_K1773003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1773003 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
no | no | ||
+ | [[File:Cas3 1.png|300px|thumb|(Restriction with EcoRI (E), XbaI (X) and PstI (P) of Wild type (WT) Cas3 gene compared to mutated Cas3 genes. The first mutant plasmid has two successful mutations, whereas the second mutant plasmid only has one successfully mutated restriction site.)|left]] | ||
Revision as of 14:01, 9 September 2015
I-F type CRISPR-Cas Cas3 gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2623
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1344
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2307
Illegal AgeI site found at 2680 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
no
Source
A.actinomycetemcomitans D7-S