Difference between revisions of "Part:BBa K1642001:Design"

 
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===References===
 
===References===
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[2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.

Revision as of 12:17, 9 September 2015

Codon-optimized Halorhodopsin with His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
    Illegal NgoMIV site found at 292
    Illegal NgoMIV site found at 613
    Illegal AgeI site found at 484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

restriction


Source

Synthesized

References

[2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.