Difference between revisions of "Part:BBa K1642004"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1642004 short</partinfo>
 
<partinfo>BBa_K1642004 short</partinfo>
 +
 
The SD-like sequence derived from the cpcB gene of Synechococcus sp. PCC7002, 5′-UAUAAGUAGGAGAUAAAAAC-3′, is to be introduced at upstream of the start codon of the genes under the PcpcG2.
 
The SD-like sequence derived from the cpcB gene of Synechococcus sp. PCC7002, 5′-UAUAAGUAGGAGAUAAAAAC-3′, is to be introduced at upstream of the start codon of the genes under the PcpcG2.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
−
===Usage and Biology===
 
−
Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence[], so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.
 
−
 
−
Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.
 
  
 
<!-- -->
 
<!-- -->

Revision as of 08:11, 9 September 2015

SD sequence of cpcB

The SD-like sequence derived from the cpcB gene of Synechococcus sp. PCC7002, 5′-UAUAAGUAGGAGAUAAAAAC-3′, is to be introduced at upstream of the start codon of the genes under the PcpcG2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]