Difference between revisions of "Part:BBa K1582004:Design"
 
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===Design Notes===
 
===Design Notes===
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We choose a linker to combine GFP with HFBI in order to prevent them from denaturation. And the amino acid sequences are GGGGSGGGGS. We have select the coding gene, which is GGTGGTGGTGGTTCTGGTGGTGGTGGTTCT
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===Source===
 
===Source===
  
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GFP comes frome iGEM's standard part and HFBI comes frome Trichoderma reesei.
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===References===
 
===References===

Latest revision as of 05:00, 9 September 2015

[[File:Media:Example.jpg]] GFP+sJanus Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 976
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI site found at 985


Design Notes

Source

References