Difference between revisions of "Part:BBa K1773003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1773003 short</partinfo> | <partinfo>BBa_K1773003 short</partinfo> | ||
− | I-F type CRISPR-Cas Cas3 protein | + | I-F type CRISPR-Cas Cas3 protein |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | This Cas3 gene has mutated EcoRI, XbaI, PstI sites for it to be biobrick compatible | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 16:43, 8 September 2015
I-F type CRISPR-Cas Cas3 gene
I-F type CRISPR-Cas Cas3 protein
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1344
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2307
Illegal AgeI site found at 2680 - 1000COMPATIBLE WITH RFC[1000]