Difference between revisions of "Part:BBa K1773019"
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<partinfo>BBa_K1773019 short</partinfo> | <partinfo>BBa_K1773019 short</partinfo> | ||
− | Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is | + | Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction. |
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Revision as of 15:47, 8 September 2015
Cas3 gene with pLux/cI right promoter and strong RBS
Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2406
Illegal AgeI site found at 2779 - 1000COMPATIBLE WITH RFC[1000]