Difference between revisions of "Part:BBa K1641002"
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This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence Rox (or its proper derivatives), which do not cross-interfering with Cre-LoxP system. Dre is one of the most widely used Cre-like recombinase. | This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence Rox (or its proper derivatives), which do not cross-interfering with Cre-LoxP system. Dre is one of the most widely used Cre-like recombinase. | ||
| − | + | Dre has the invertase activity that invert the the sequence between two anti-parallel Roxs. Dre is a integrase used by P1-like coliphage, yet this coding sequence is not the wild type sequence but redesigned into E. coli-and-yeast coding preference according to its AA sequence. | |
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Latest revision as of 09:07, 8 September 2015
Recombinase Dre, E. coli and yeast coding optimized
This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence Rox (or its proper derivatives), which do not cross-interfering with Cre-LoxP system. Dre is one of the most widely used Cre-like recombinase.
Dre has the invertase activity that invert the the sequence between two anti-parallel Roxs. Dre is a integrase used by P1-like coliphage, yet this coding sequence is not the wild type sequence but redesigned into E. coli-and-yeast coding preference according to its AA sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]