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	To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 1 genes. This gene is taken from the genome <i>Apergillus terreus</i>. It is coding for a cis-aconitate decarboxylase (cadA). An Enzyme that catalyzes the following chemical reaction:		To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 1 genes. This gene is taken from the genome <i>Apergillus terreus</i>. It is coding for a cis-aconitate decarboxylase (cadA). An Enzyme that catalyzes the following chemical reaction:
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	<img style="width: 900px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src=" https://static.igem.org/mediawiki/2015/a/ae/Itaconic_acid_chemistry.jpg">		<img style="width: 900px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src=" https://static.igem.org/mediawiki/2015/a/ae/Itaconic_acid_chemistry.jpg">

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Revision as of 09:03, 8 September 2015

Inducible itaconic acid producing operon (only cadA)

Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized most commonly in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation.
To enable this pathway in Escherichia coli it is necessary to introduce 1 genes. This gene is taken from the genome Apergillus terreus. It is coding for a cis-aconitate decarboxylase (cadA). An Enzyme that catalyzes the following chemical reaction:

Figure 1 Reaction scheme of the itaconic acid producing operon (only cadA). The substrate for the reaction is cis-aconitate. Cis-aconitate is metabolized to itaconic acid in 1 step by decarboxylation.

Usage

This part is a composite of one coding genes, provided with a strong RBS (BBa_B0034) and under control of a T7 Promoter (BBa_I719005). Optimization of this operon may be possible through introduction of 2 more genes. Namely gltA (Part:BBa_K1602001) and acnA (Part:BBa_K1602002).

cis-aconitate decarboxylase - cadA (BBa_K1602003)
	Figure 2 Genetic map of the itaconic acid producing operon (only cadA) with T7 promoter. This brick enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.

Sequence and Features