Difference between revisions of "Part:BBa K1666003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The lsrD gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating). | + | The ''lsrD'' gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating). |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene | + | We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene ''lsrD''(Gene ID: 1255603). |
===References=== | ===References=== | ||
+ | [1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in ''Salmonella typhimurium''. Mol Microbiol, 2001, 42(3): 777–793 | ||
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+ | [2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396 | ||
+ | |||
+ | [3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in ''Salmonella typhimurium''. Mol Microbiol, 2003, 50(4): 1411-1427. |
Latest revision as of 14:49, 6 September 2015
LsrD of LuxS/AI-2 signaling pathway in Salmonalla
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 747
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lsrD gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
Source
We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene lsrD(Gene ID: 1255603).
References
[1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol Microbiol, 2001, 42(3): 777–793
[2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396
[3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol, 2003, 50(4): 1411-1427.