Difference between revisions of "Part:BBa K1699002:Design"

(→‎Design Notes)
(→‎References)
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===References===
 
===References===
  
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1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
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1. In vivo genome editing using Staphylococcus aureus Cas9.
−
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full
+
<br />10.1038/nature14299
 +
 
 +
2. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
 +
<br />http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full

Revision as of 19:30, 5 September 2015

gRNA for SaCas9 targeting human ubiquitin B gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSB1C3 using EcoRI and PstI restriction sites.
F. tagtcatgGAATTCGCGGCCGCTTCTAG
R. tgtatactCTGCAGCGGCCGCTACTAG

Source

IDT de-novo synthesis.

References

1. In vivo genome editing using Staphylococcus aureus Cas9.
10.1038/nature14299

2. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full