Difference between revisions of "Part:BBa K1699005"

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===Charactarization===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 19:25, 5 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6(RNA pol III) promoter.


Usage and Biology

gRNA is part of CRISPR/Cas9 complex. MLP is a synthetic promoter with three complementing sites for the gRNA.


Charactarization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]