Difference between revisions of "Part:BBa K1699003:Design"

(→‎Source)
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Design
 
−
 
  
 +
Cloned into pSBC13 using EcoRI and PstI restriction sites.
 +
<br /> F. tagtcatgGAATTCGCGGCCGCTTCTAG
 +
<br /> R. tgtatactCTGCAGCGGCCGCTAC
  
 
===Source===
 
===Source===

Revision as of 19:16, 5 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSBC13 using EcoRI and PstI restriction sites.
F. tagtcatgGAATTCGCGGCCGCTTCTAG
R. tgtatactCTGCAGCGGCCGCTAC

Source

IDT de-novo synthesis.

References

1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full

2. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas http://pubs.acs.org/doi/full/10.1021/sb400081r