Difference between revisions of "Part:BBa K1699002:Design"
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Revision as of 19:05, 5 September 2015
gRNA for SaCas9 targeting human ubiquitin B gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 146
Illegal NgoMIV site found at 175 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Cloned into pSB1C3 using EcoRI and PstI restriction sites.
Source
IDT de-novo synthesis.
References
1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full