Difference between revisions of "Part:BBa K1699001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | hTERT promotes the transcription of the catalytic subunit of telomerase. Telomerase elognates the ends of chromosomes, regions called telomers. hTERT is not active in somatic cells and appears to be highly active in most if not all cancer cells, therefor, cells that will express an exogenic gene under hTERT are likely to be cancerous. | ||
| + | We have used this promoter as part of a two promoter system, in order to detect whether a cell was a cancer cell. | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 18:57, 5 September 2015
Human short TERT promoter
Human short TERT promoter.
hTERT (human Telomerase Reverse Transcriptase) is a human promoter which controls the transcription of Telomerase, a gene highly expressed in cancer cells, and not in healthy ones (1).
Usage and Biology
hTERT promotes the transcription of the catalytic subunit of telomerase. Telomerase elognates the ends of chromosomes, regions called telomers. hTERT is not active in somatic cells and appears to be highly active in most if not all cancer cells, therefor, cells that will express an exogenic gene under hTERT are likely to be cancerous. We have used this promoter as part of a two promoter system, in order to detect whether a cell was a cancer cell.
Characterization
characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]