Difference between revisions of "Part:BBa K1824002"

Latest revision as of 17:11, 5 September 2015

Constitutive Promoter J23119 + RNA Thermometer U6

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer U6 BBa_K1824562 for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT U6.

Characterization

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 60
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 60
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 46
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 60
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 60
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1824002 short</partinfo>
-This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer U6 BBa_K1824562 for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
+This is a composite regulatory part that consisting of constitutive promoter <partinfo>J23119</partinfo> and RNA Thermometer U6 <partinfo>BBa_K1824562</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
+[[Image:U6.jpg|360px]]
+<br>'''Figure 1:''' Possible secondary structure of RNAT U6.
+===Characterization===
-<!-- Add more about the biology of this part here
 ===Usage and Biology===