Difference between revisions of "Part:BBa K1824001"

 
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<partinfo>BBa_K1824001 short</partinfo>
 
<partinfo>BBa_K1824001 short</partinfo>
  
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This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer FourU for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
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This is a composite regulatory part that consisting of constitutive promoter <partinfo>J23119</partinfo> and RNA Thermometer FourU for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
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[[Image:XJTLU-FourU.jpg|450px]]
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<br>'''Figure 1:''' Possible secondary structure of RNAT FourU.
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===Characterization===
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 15:53, 5 September 2015

Constitutive Promoter J23119 + RNA Thermometer FourU

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer FourU for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


XJTLU-FourU.jpg
Figure 1: Possible secondary structure of RNAT FourU.

Characterization

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]