Difference between revisions of "Part:BBa K1766001"

Revision as of 15:26, 5 September 2015

MicF Target

The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].

BBa_K1766001 consists of the micF target under the control of Anderson promoter BBa_J23101.

The micF target can be fused to a protein of interest. It is then possible to down-regulate protein expression using MicF BBa_K176600.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

@@ Line 7: / Line 7: @@
 BBa_K1766001 consists of the micF target under the control of Anderson promoter BBa_J23101.
-The micF target can be fused to a protein of interest. It is then possible to down-regulate protein expression using MicF (BBa_K176600).
+The micF target can be fused to a protein of interest. It is then possible to down-regulate protein expression using MicF [[BBa_K176600]].
@@ Line 14: / Line 14: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'>'''Sequence and Features'''</span>
 <partinfo>BBa_K1766001 SequenceAndFeatures</partinfo>