Difference between revisions of "Part:BBa K1620007"

 
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SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick BBa_K519010 originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli.
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1620007 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1620007 parameters</partinfo>
 
<partinfo>BBa_K1620007 parameters</partinfo>
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==Usage and Biology==
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SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick <partinfo>BBa_K519010</partinfo> originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.
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[[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|left|'''Figure 1:''' Alignment between improved SmtA and Bba_K519010.]]
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Revision as of 13:20, 5 September 2015

Synechococcus metallothionenin A (SmtA)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick BBa_K519010 originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.

Figure 1: Alignment between improved SmtA and Bba_K519010.