Difference between revisions of "Part:BBa K1824000:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1824000 short</partinfo>
 
<partinfo>BBa_K1824000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. it is important to make sure that those redundant sequences do not interact with RNA thermometers.
+
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence.  
  
  

Latest revision as of 11:33, 5 September 2015

Constitutive Promoter J23119 + RNA Thermometer A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence.


Source

J23119 was from the Registry, and A1 was from BIT-CHINA.

References