Difference between revisions of "Part:BBa K1699005"

(Usage and Biology)
Line 2: Line 2:
 
<partinfo>BBa_K1699005 short</partinfo>
 
<partinfo>BBa_K1699005 short</partinfo>
  
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
+
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6(RNA pol III) promoter.
  
  

Revision as of 19:10, 4 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6(RNA pol III) promoter.


Usage and Biology

gRNA is part of CRISPR/Cas9 complex. MLP is a synthetic promoter with three complementing sites for the gRNA.

Characterization

characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]