Difference between revisions of "Part:BBa K1720001:Design"

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===Design Notes===
 
===Design Notes===
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This part do not contains promoter so that users can add their own promotor vary from different purposes.
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The vector we used in our experiment was created using scarless golden gate assembly.We cloned this basic part into a psb1c3 vector without promoter , so that user can choose their own promoter for different purpose. After standardizing our part, a promoter can be easily inserted in front of our part. This part is only a subunit of human guanylate cyclase,.To form holoenzyme this part needs to work with BBa_K1720000 together.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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1. Gheorghiade M, Marti CN, Sabbah HN, Roessig L, Greene SJ, Bohm M, Burnett JC, Campia U, Cleland JG, Collins SP et al: Soluble  guanylate cyclase: a potential therapeutic target for heart failure. Heart failure reviews 2013, 18(2):123-134.
 +
 +
2. Sharina IG, Cote GJ, Martin E, Doursout MF, Murad F: RNA splicing in regulation of nitric oxide receptor soluble guanylyl cyclase. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society 2011, 25(3):265-274.
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 +
3. Marinko M, Novakovic A, Nenezic D, Stojanovic I, Milojevic P, Jovic M, Ugresic N, Kanjuh V, Yang Q, He GW: Nicorandil directly and cyclic GMP-dependently opens K channels in human bypass grafts. Journal of pharmacological sciences 2015.

Latest revision as of 07:32, 4 September 2015


Human guanylate cyclase1,soluble, beta 3 unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 579
    Illegal BamHI site found at 800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The vector we used in our experiment was created using scarless golden gate assembly.We cloned this basic part into a psb1c3 vector without promoter , so that user can choose their own promoter for different purpose. After standardizing our part, a promoter can be easily inserted in front of our part. This part is only a subunit of human guanylate cyclase,.To form holoenzyme this part needs to work with BBa_K1720000 together.

Source

We get the informations from this link: http://www.ncbi.nlm.nih.gov/gene/2983

References

1. Gheorghiade M, Marti CN, Sabbah HN, Roessig L, Greene SJ, Bohm M, Burnett JC, Campia U, Cleland JG, Collins SP et al: Soluble guanylate cyclase: a potential therapeutic target for heart failure. Heart failure reviews 2013, 18(2):123-134.

2. Sharina IG, Cote GJ, Martin E, Doursout MF, Murad F: RNA splicing in regulation of nitric oxide receptor soluble guanylyl cyclase. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society 2011, 25(3):265-274.

3. Marinko M, Novakovic A, Nenezic D, Stojanovic I, Milojevic P, Jovic M, Ugresic N, Kanjuh V, Yang Q, He GW: Nicorandil directly and cyclic GMP-dependently opens K channels in human bypass grafts. Journal of pharmacological sciences 2015.