Difference between revisions of "Part:BBa K1618027"
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Revision as of 15:30, 3 September 2015


GlgB-Rv3037c with IPTG-inducible promoter

Rv3037c encodes for a putative acyltransferase enzymes which can acylate glycogen/starch. This is preceded by GlgB which codes for a glycogen branching enzyme which cleaves alpha-1,4 linkages in glycogen and re-anneals the cleaved strand onto the main glycogen strand to create an alpha-1,6 linkage to create a branch. Promoted by an IPTG-inducible promoter and encoded within the standard pSB1C3 vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 3142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2952
    Illegal NgoMIV site found at 3125
    Illegal NgoMIV site found at 3449
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917