Difference between revisions of "Part:BBa K1699002"
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gRNA for SaCas9 targeting ubiquitin B
 
gRNA for SaCas9 targeting ubiquitin B
  
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Ribozyme flanked gRNA compatible for SaCas9. gRNA sequence complements 3 different loci in the second exon of human UBB gene. It has a hammerhead ribozyme on its 5' and a
  
 
===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K1699002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1699002 SequenceAndFeatures</partinfo>
  
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===References===
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6. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
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http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 08:17, 3 September 2015

gRNA for SaCas9 targeting human ubiquitin B gene

gRNA for SaCas9 targeting ubiquitin B

Ribozyme flanked gRNA compatible for SaCas9. gRNA sequence complements 3 different loci in the second exon of human UBB gene. It has a hammerhead ribozyme on its 5' and a

Usage and Biology

usage and biology


Characterization

characterization


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]

References

6. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full