text-align:justify "><em><strong>FIGURE 3.</strong> ''' Induced and not induced culture growth in MOPS mediums.'''<br/> NEB10beta cells, tranformed with part BBa_K731250 (in pSB3C5) were grown in LB broth until OD_600= 0.3 then centrifuged and resuspended in equal amount of minimal MOPS medium and induced at OD_600 = 0.6 with 5mM arabinose concentration. On the left OD mesurements of coltures growing in minimal MOPS A medium (glycerol, 60mM). In orange not induced culture, in blue induced (arabinose 5mM). Same colors are used on the chart on the right for induced-not induced bacteria with MOPS B medium (30 mM glucose). Inhibition of growth is observed in MOPS A when induced. There seems to be no effect in MOPS B. This particular behaviour is probably due to energy assessment while growing on glycerol as the sole carbon source. More energy is invested in protein production as observed in FIGURE 2. </em> </p>
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Revision as of 15:16, 1 September 2015
J23100 + β-carotene 15,15’-dioxygenase (blh gene)
blh encodes for β-carotene 15,15’-dioxygenase, the enzyme that cleaves one molecule of β-carotene into two molecules of retinal
Usage and Biology
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7 Illegal NheI site found at 30
