Difference between revisions of "Part:BBa K1585103:Design"
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<partinfo>BBa_K1585103 short</partinfo> | <partinfo>BBa_K1585103 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. | We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. | ||
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[[File:K15751xx design.png |600px]] | [[File:K15751xx design.png |600px]] | ||
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===Source=== | ===Source=== | ||
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Assambly of [https://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [https://parts.igem.org/Part:BBa_R0010 R_0010] | Assambly of [https://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [https://parts.igem.org/Part:BBa_R0010 R_0010] | ||
Latest revision as of 09:04, 1 September 2015
Anderson Promoter with lacI binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone J61002 because the functionality is directly visible by adding IPTG. RFP (E1010) should get expressed then.
Source
Assambly of J23103 and lacI-binding-site of R_0010
The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207.