Difference between revisions of "Part:BBa K1598002:Design"

(→‎Source)
(→‎Design Notes)
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1598002 short</partinfo>
 
<partinfo>BBa_K1598002 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Codon optimized for E.coli  
+
<html>
−
 
+
- Lac induced expression cassette (<a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a>) was transformed from 2014 iGEM distribution <br>
−
 
+
- TPH1 human gene was codon optimized for <i>E.coli</i>, synthesized and submitted as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001">BBa_K1598001</a><br>
 +
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together<br><br>
 +
<img src="https://static.igem.org/mediawiki/2015/6/6d/UCL_BBaJ045440x4%2BTPH1Expcasettex5-22Jul15-2.png" style="width: 500px;"><br>
 +
</html>
  
 
===Source===
 
===Source===
 +
  
−
- Lac induced expression cassette (<a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a>) was transformed from 2014 iGEM distribution <br>
+
DNA synthesis
−
- TPH1 human gene was codon optimized for <i>E.coli</i>, synthesized and submitted as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001">BBa_K1598001</a><br>
+
−
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/6/6d/UCL_BBaJ045440x4%2BTPH1Expcasettex5-22Jul15-2.png">
+
  
 
===References===
 
===References===

Latest revision as of 20:17, 27 August 2015

Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- Lac induced expression cassette (BBa_K314103) was transformed from 2014 iGEM distribution
- TPH1 human gene was codon optimized for E.coli, synthesized and submitted as BBa_K1598001
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together


Source

DNA synthesis

References