Difference between revisions of "Part:BBa K1598002:Design"
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===Source=== | ===Source=== | ||
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- Lac induced expression cassette (<a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a>) was transformed from 2014 iGEM distribution <br> | - Lac induced expression cassette (<a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a>) was transformed from 2014 iGEM distribution <br> | ||
- TPH1 human gene was codon optimized for <i>E.coli</i>, synthesized and submitted as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001">BBa_K1598001</a><br> | - TPH1 human gene was codon optimized for <i>E.coli</i>, synthesized and submitted as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001">BBa_K1598001</a><br> | ||
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together<br> | - parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together<br> | ||
<img src="https://static.igem.org/mediawiki/2015/6/6d/UCL_BBaJ045440x4%2BTPH1Expcasettex5-22Jul15-2.png"> | <img src="https://static.igem.org/mediawiki/2015/6/6d/UCL_BBaJ045440x4%2BTPH1Expcasettex5-22Jul15-2.png"> | ||
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===References=== | ===References=== | ||
Revision as of 20:02, 27 August 2015
Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for E.coli
Source
- Lac induced expression cassette (BBa_K314103) was transformed from 2014 iGEM distribution
- TPH1 human gene was codon optimized for E.coli, synthesized and submitted as BBa_K1598001
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together
