Difference between revisions of "Part:BBa K1598002:Design"

 
(→‎Source)
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===Source===
 
===Source===
  
−
Lac induced expression cassette from 2014 iGEM distribution
+
- Lac induced expression cassette (<a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a>) was transformed from 2014 iGEM distribution <br>
−
Tryptophan hydroxylase 1 - TPH1 human gene, synthesized  
+
- TPH1 human gene was codon optimized for <i>E.coli</i>, synthesized and submitted as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001">BBa_K1598001</a><br>
 +
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together<br>
 +
<img src="https://static.igem.org/mediawiki/2015/6/6d/UCL_BBaJ045440x4%2BTPH1Expcasettex5-22Jul15-2.png">
  
 
===References===
 
===References===

Revision as of 20:01, 27 August 2015


Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E.coli


Source

- Lac induced expression cassette (<a href="https://parts.igem.org/Part:BBa_K314103">BBa_K314103</a>) was transformed from 2014 iGEM distribution
- TPH1 human gene was codon optimized for E.coli, synthesized and submitted as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001">BBa_K1598001</a>
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together
<img src="UCL_BBaJ045440x4%2BTPH1Expcasettex5-22Jul15-2.png">

References