Difference between revisions of "Part:BBa I13453:Experience"
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| + | <partinfo>BBa_I13453 AddReview 5</partinfo> | ||
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| + | '''pBad I13453''' confers expression of tetracycline resistance when placed upstream of '''hixC-RBS-Tet...''' (<partinfo>BBa_J44010</partinfo>). The presence of '''hixC''' (26 bp) between '''pBad''' and '''RBS-Tet''' does not disrupt transcription. In the insulated vector <partinfo>pSB1A7</partinfo> we have observed that tetracycline resistance depends upon the presence and proper orientation of '''pBad''' relative to the coding region (Davidson and Missouri Western 2006). '''pBad-hixC-RBS-Tet''' is tetracycline resistant, whereas '''RBS-Tet''' and '''pBad-hixC-Tet<sub>rev</sub>-RBS<sub>rev</sub>''' are not. We have observed Tet expression in the absence of L-arabinose (the affects of L-arabinose were not tested in this system). In summary, '''pBad I13453''' (which includes the Cap and AraC protein binding domains and polymerase binding site; Lee, PNAS, 1981) appears to be a functional constitutive promoter. | ||
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Revision as of 01:07, 8 December 2006
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_I13453
User Reviews
UNIQ08bb79ab461e0373-partinfo-00000000-QINU
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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kahaynes |
pBad I13453 confers expression of tetracycline resistance when placed upstream of hixC-RBS-Tet... (BBa_J44010). The presence of hixC (26 bp) between pBad and RBS-Tet does not disrupt transcription. In the insulated vector pSB1A7 we have observed that tetracycline resistance depends upon the presence and proper orientation of pBad relative to the coding region (Davidson and Missouri Western 2006). pBad-hixC-RBS-Tet is tetracycline resistant, whereas RBS-Tet and pBad-hixC-Tetrev-RBSrev are not. We have observed Tet expression in the absence of L-arabinose (the affects of L-arabinose were not tested in this system). In summary, pBad I13453 (which includes the Cap and AraC protein binding domains and polymerase binding site; Lee, PNAS, 1981) appears to be a functional constitutive promoter. |
UNIQ08bb79ab461e0373-partinfo-00000005-QINU