Difference between revisions of "Part:BBa K1720001:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We used lentiviral vector to transfected alpha 3 unit and beta 3 unit to HEK293 cells together. Since soluble guanylate cyclases (sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP(cGMP) and pyrophosphate. The level of cGMP will be up regulated.The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level.
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This part do not contains promoter so that users can add their own promotor vary from different purposes.
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===Source===
 
===Source===

Revision as of 08:10, 26 August 2015


Human guanylate cyclase1,soluble, beta 3 unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 579
    Illegal BamHI site found at 800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part do not contains promoter so that users can add their own promotor vary from different purposes.

Source

We get the informations from this link: http://www.ncbi.nlm.nih.gov/gene/2983

References