Difference between revisions of "Part:BBa K1720000:Design"

 
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<partinfo>BBa_K1720000 short</partinfo>
 
<partinfo>BBa_K1720000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This subunit coding part with a GFP reporter was then transfected into HEK293 cells and in vivo green fluorescence signal was observed under fluorescence microscope. After that we used lentiviral vector to transfected alpha 3 unit and beta 3 unit to HEK293 cells together. Since soluble guanylate cyclases (sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP(cGMP) and pyrophosphate. The level of cGMP will be up regulated.The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level.  
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This part do not contains promoter so that users can add their own promoter vary from different purpose.
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===References===
 
===References===
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1. Gheorghiade M, Marti CN, Sabbah HN, Roessig L, Greene SJ, Bohm M, Burnett JC, Campia U, Cleland JG, Collins SP et al: Soluble guanylate cyclase: a potential therapeutic target for heart failure. Heart failure reviews 2013, 18(2):123-134.
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2. Sharina IG, Cote GJ, Martin E, Doursout MF, Murad F: RNA splicing in regulation of nitric oxide receptor soluble guanylyl cyclase. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society 2011, 25(3):265-274.
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3. Marinko M, Novakovic A, Nenezic D, Stojanovic I, Milojevic P, Jovic M, Ugresic N, Kanjuh V, Yang Q, He GW: Nicorandil directly and cyclic GMP-dependently opens K channels in human bypass grafts. Journal of pharmacological sciences 2015.

Revision as of 05:47, 26 August 2015

Human guanylate cyclase1,soluble, alpha 3 unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1576
    Illegal SapI site found at 1446


Design Notes

This part do not contains promoter so that users can add their own promoter vary from different purpose.


Source

http://www.ncbi.nlm.nih.gov/gene/2982

References

1. Gheorghiade M, Marti CN, Sabbah HN, Roessig L, Greene SJ, Bohm M, Burnett JC, Campia U, Cleland JG, Collins SP et al: Soluble guanylate cyclase: a potential therapeutic target for heart failure. Heart failure reviews 2013, 18(2):123-134. 2. Sharina IG, Cote GJ, Martin E, Doursout MF, Murad F: RNA splicing in regulation of nitric oxide receptor soluble guanylyl cyclase. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society 2011, 25(3):265-274. 3. Marinko M, Novakovic A, Nenezic D, Stojanovic I, Milojevic P, Jovic M, Ugresic N, Kanjuh V, Yang Q, He GW: Nicorandil directly and cyclic GMP-dependently opens K channels in human bypass grafts. Journal of pharmacological sciences 2015.