Difference between revisions of "Part:BBa K1403012:Design"
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<partinfo>BBa_K1403012 short</partinfo> | <partinfo>BBa_K1403012 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The purpose of this part is to biosynthesize isoamyl acetate in <i>E.coli</i>. | |
| + | We used the ATF1 generator <partinfo>BBa_J45199</partinfo> and inserted a constitutive promoter <partinfo>BBa_J23108</partinfo> Alcohol acetyltransferase I converts isoamyl alcohol to isoamyl acetate, which has a banana smell. The gBlock and linearized pSB1C3 vector were amplified by PCR, digested and ligated. The finished plasmid is in standard BioBrick format. | ||
| + | Constitutive promoter <partinfo>BBa_J23108</partinfo> has a relative activity of 0.51. | ||
===Source=== | ===Source=== | ||
| + | <partinfo>BBa_J45199</partinfo> | ||
| + | ATF1 generator, the CDS comes from <i>Saccharomyces cerevisiae</i>. | ||
| − | + | ===References=== | |
| + | [https://parts.igem.org/Promoters/Catalog/Anderson Anderson Promoter Collection] | ||
| − | + | [https://parts.igem.org/Part:BBa_J45199 iGEM 2006 MIT] | |
Latest revision as of 12:28, 25 August 2015
Alcohol acetyltransferase I (ATF1) expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 163
Illegal BamHI site found at 1490 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 590
Illegal SapI.rc site found at 1598
Design Notes
The purpose of this part is to biosynthesize isoamyl acetate in E.coli.
We used the ATF1 generator BBa_J45199 and inserted a constitutive promoter BBa_J23108 Alcohol acetyltransferase I converts isoamyl alcohol to isoamyl acetate, which has a banana smell. The gBlock and linearized pSB1C3 vector were amplified by PCR, digested and ligated. The finished plasmid is in standard BioBrick format.
Constitutive promoter BBa_J23108 has a relative activity of 0.51.
Source
BBa_J45199 ATF1 generator, the CDS comes from Saccharomyces cerevisiae.