Difference between revisions of "Part:BBa J06504:Design"

(Source)
 
(One intermediate revision by the same user not shown)
Line 19: Line 19:
 
===Source===
 
===Source===
  
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
+
mCherry from Roger Y. Tsien's lab, altered to be BioBrick-compatible.
  
 
===References===
 
===References===
 +
See main page.

Latest revision as of 19:26, 1 December 2006


monomeric RFP optimized for bacteria


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.


Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.



Source

mCherry from Roger Y. Tsien's lab, altered to be BioBrick-compatible.

References

See main page.