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===Design note=== | ===Design note=== | ||
Latest revision as of 06:02, 24 August 2015
The coding sequence of the SCFV of anti-p185her2/neu antibody chA21
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 370
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
This coding sequence was synthesized by LCR assmebly using 36 oilgos
Design note
Isolation:
After the LCR assembly, we isolated the CDS using these two primers(with BioBrick Prefix in the fwd primer and Suffix in the rev primer):
fwd:GAATTCGCGGCCGCTTCTAGatgGGTTCTACTCAAGttt
rev:TACTAGTAGCGGCCGCTGCAGAGATTGACAATCTTCAGAAGAT
The termination codon:
Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.
References
[1]Zhou H, Zha Z, Liu Y, et al. Structural Insights into the Down-regulation of Overexpressed p185her2/neu Protein of Transformed Cells by the Antibody chA21[J]. Journal of Biological Chemistry, 2011, 286(36): 31676-31683.