Difference between revisions of "Part:BBa K1607010:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1607010 short</partinfo>
 
<partinfo>BBa_K1607010 short</partinfo>
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<partinfo>BBa_K1607010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1607010 SequenceAndFeatures</partinfo>
  
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===Source===
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This coding sequence was synthesized by LCR assmebly using 36 oilgos
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[[see oligos and protocols]]
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===Design note===
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'''Isolation:'''
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After the LCR assembly, we isolated the CDS using these two primers(with BioBrick Prefix in the fwd primer and Suffix in the rev primer):
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fwd:GAATTCGCGGCCGCTTCTAGatgGGTTCTACTCAAGttt
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rev:TACTAGTAGCGGCCGCTGCAGAGATTGACAATCTTCAGAAGAT
  
 
'''The termination codon:'''
 
'''The termination codon:'''
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    Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.
 
  
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===Source===
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Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.
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    This coding sequence is synthesized by LCR assmebly using 36 oilgos.
+
  
 
===References===
 
===References===
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[1]Zhou H, Zha Z, Liu Y, et al. Structural Insights into the Down-regulation of Overexpressed p185her2/neu Protein of Transformed Cells by the Antibody chA21[J]. Journal of Biological Chemistry, 2011, 286(36): 31676-31683.

Latest revision as of 06:02, 24 August 2015

The coding sequence of the SCFV of anti-p185her2/neu antibody chA21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 370
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

This coding sequence was synthesized by LCR assmebly using 36 oilgos

see oligos and protocols

Design note

Isolation:

After the LCR assembly, we isolated the CDS using these two primers(with BioBrick Prefix in the fwd primer and Suffix in the rev primer):

fwd:GAATTCGCGGCCGCTTCTAGatgGGTTCTACTCAAGttt

rev:TACTAGTAGCGGCCGCTGCAGAGATTGACAATCTTCAGAAGAT

The termination codon:

Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.

References

[1]Zhou H, Zha Z, Liu Y, et al. Structural Insights into the Down-regulation of Overexpressed p185her2/neu Protein of Transformed Cells by the Antibody chA21[J]. Journal of Biological Chemistry, 2011, 286(36): 31676-31683.