Difference between revisions of "Part:BBa K1758120:Design"
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*Amplification of the pSB1C3-Backbone: | *Amplification of the pSB1C3-Backbone: | ||
| − | '''Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre''' | + | '''Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre]''' |
| − | '''Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf''' | + | '''Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf]''' |
Revision as of 23:39, 21 August 2015
RNase E regulating protein RraA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amplification with Gibson-primer:
- Amplification of regulator of ribonuclease activity A (RraA):
Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_fwd RraA_fwd]
Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_rev RraA_rev]
- Amplification of the pSB1C3-Backbone:
Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre]
Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf]
Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].
Source
RraA was obtained via PCR on E. coli K12 genomic DNA.