Difference between revisions of "Part:BBa C0040:Design"
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Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999).   
 
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999).   
  
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Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630   
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Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630   
  
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References (unparsed) here:  
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References (unparsed) here:  
  
 
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999).   
 
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999).   
  
−
Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630   
+
Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630   
  
 
BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator.      It has been modified to include a rapid degradation LVA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.   
 
BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator.      It has been modified to include a rapid degradation LVA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.   
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Revision as of 01:49, 30 April 2006


tetracycline repressor from transposon Tn10 (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

References (unparsed) here:

Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).

Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630

References (unparsed) here:

Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).

Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630

BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.


Source

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References

Files

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