Difference between revisions of "Part:BBa K1638018"

Revision as of 23:11, 15 August 2015

hTrx-based scaffold fused to T18 through a flexible linker

This composite part consists of the hTrx-scaffold used for presenting peptide aptamers fused to the T18 domain of CyaA as used in the bacterial two-hybrid system. This part is used in bacterial two-hybrid screening for peptide aptamers against specific targets. When a target candidate is fused to the T25 domain of CyaA, and a peptide aptamer binds the target, the T18 and T25 domain is brought into proximity of one another and the formation of cAMP is induced. When combined with a cAMP reporter system, like a system promoting the transcription of RFP, the presence of red-flourescent bacteria indicates an interaction between the peptide aptamer and the target protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 624
Illegal XhoI site found at 753
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 165
Illegal NgoMIV site found at 575
Illegal AgeI site found at 381
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1638018 short</partinfo>
-coming
+This composite part consists of the hTrx-scaffold used for presenting peptide aptamers fused to the T18 domain of CyaA as used in the bacterial two-hybrid system. This part is used in bacterial two-hybrid screening for peptide aptamers against specific targets. When a target candidate is fused to the T25 domain of CyaA, and a peptide aptamer binds the target, the T18 and T25 domain is brought into proximity of one another and the formation of cAMP is induced. When combined with a cAMP reporter system, like a system promoting the transcription of RFP, the presence of red-flourescent bacteria indicates an interaction between the peptide aptamer and the target protein.
 <!-- Add more about the biology of this part here