Difference between revisions of "Part:BBa K1582004:Design"
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===Design Notes===
 
===Design Notes===
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When amplify the HFBI and GFP with PCR, temperature is important.
+
We choose a linker to combine GFP with HFBI in order to prevent them from denaturation. And the amino acid sequences are GGGGSGGGGS. We have select the coding gene, which is GGTGGTGGTGGTTCTGGTGGTGGTGGTTCT
  
  

Revision as of 02:28, 8 August 2015

[[File:Media:Example.jpg]] GFP+sJanus Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 976
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI site found at 985


Design Notes

We choose a linker to combine GFP with HFBI in order to prevent them from denaturation. And the amino acid sequences are GGGGSGGGGS. We have select the coding gene, which is GGTGGTGGTGGTTCTGGTGGTGGTGGTTCT


Source

GFP comes frome iGEM's standard part and HFBI comes frome Trichoderma reesei.

References