Difference between revisions of "Part:BBa K1582004:Design"

 
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Revision as of 09:06, 7 August 2015

[[File:Media:Example.jpg]] GFP+sJanus Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 976
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI site found at 985


Design Notes

When amplify the HFBI and GFP with PCR, temperature is important.


Source

GFP comes frome iGEM's standard part and HFBI comes frome Trichoderma reesei.

References