Difference between revisions of "Part:BBa B0012:Design"
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Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The      5' end cutoff was placed immediately after the TAA stop codon and the 3' end      cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).
 
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The      5' end cutoff was placed immediately after the TAA stop codon and the 3' end      cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).
  
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Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
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Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
  
  

Revision as of 01:49, 30 April 2006


TE from coliphageT7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).

Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.


Source

--What is the source of this part?--

References

Files