Difference between revisions of "Part:BBa K1648001"

 
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[[File:1.2.C3 mamXY mamGFDC mms6 Recombination Map.png]]__NOTOC__
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<partinfo>BBa_K1648001 short</partinfo>
 
<partinfo>BBa_K1648001 short</partinfo>
  

Revision as of 16:30, 14 July 2015

1.2.C3 mamXY mamGFDC mms6 Recombination Map.png Recombination Template for mamXY, mamGC and mms Operons

This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1333
    Illegal BamHI site found at 432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 603
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 875
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 470
    Illegal BsaI site found at 569
    Illegal BsaI site found at 850